The Vancouver 2010 champions kept their French title rivals Gabriella Papadams and Guillaume Ciceronize at bay with a score of 83.67.
That bettered their own previous record of 82.68, and put them 1.64 points clear of their younger training partners Papadams and Ciceronize going into Tuesday's free dance final.
Despite their show-stopping samba rhumba cha performance a third Olympic title is by no means in the bag for Scott and Moir as Papadams and Ciceronize could overtake them if they produce their best free dance.
After claiming gold on home ice in 2010 Virtue and Moir missed out on the title at Sochi four years ago, having to settle for silver.
They also picked up team silver in Russia, but turned that into gold when helping the Canadians to go one better in Nonchanging last week.
The couple, who joined forces in 1997, took a two year time out after Sochi, changed coaches, and returned with a bang to beat Papadams and Ciceronize in last years world championships in Helsinki.
Placed third after the short programme were Madison Hubbell and Zachary Donohue, the Americans narrowly ahead of their compatriots, brother and sister Maia and Alex Shibuichi.
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