Tahrir al-Sham, led by the jihadist Fateh al-Sham Front, made the threat late Tuesday in response to an agreement signed by rebel backer Turkey and regime supporters Iran and Russia in Kazakhstan last week.
The agreement calls for the establishment of four "de- escalation zones" where rebels and government forces will halt hostilities, including air strikes, for six months.
But it carves out an exception for the continued targeting of jihadist groups like the Islamic State group and Fateh al-Sham, previously known as Al-Nusra Front.
It called on its allies to "fight any criminal gangs and spend every effort to prevent" rebel groups trying to advance on its territory.
Neither Syria's government nor the rebels are direct signatories to the Astana deal. While the foreign ministry in Damascus has said it accepts the agreement, the opposition has expressed concerns.
The exact borders of the de-escalation zones are to be delineated by June 4, but a ceasefire in the rough areas affected came into effect on Friday night.
But Fateh al-Sham is a powerful force and a regular ally for rebels in several opposition-controlled areas, particularly in Idlib.
Analysts expect the close relationship will complicate the implementation of the deal, which was reached in Astana during a fresh round of talks on Syria's war brokered by Ankara, Moscow, and Tehran.
Under the agreement, the three powers are responsible for "separating" jihadist groups like IS and Fateh al-Sham from other rebel factions.
The conflict broke out with protests in 2011 against the Damascus government, but has since evolved into a multi-front war that has killed more than 320,000 people.
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