The former King Albert II of the Belgians submitted to a DNA test on Tuesday in order avoid daily fines of 5,000 euros (USD 5,600), his lawyers said.
The test is a major moment in the long battle between Albert II and sculptor Delphine Boel, who launched proceedings before a top Brussels court in 2013 to have Albert's paternity recognised.
"After having taken note of the judgment (on daily fines) handed down on May 16 by the Brussels Court of Appeal... HM King Albert decided to submit to the expertise ordered by the Court of Appeal," his lawyers said in a statement.
The former sovereign carried out the test on Tuesday morning, one of them told AFP.
Boel, born in 1968, claims she is the child of a long affair between her mother, Sibylle de Selys Longchamps and the then crown prince Albert, married since 1959 to Paola Ruffo di Calabria.
The 81-year-old former monarch, who reigned from 1993 until 2013, has always refused to acknowledge that he could be her father.
Last October, the Brussels Court of Appeal ruled in favour of Boel and ordered Albert II to submit to a genetic sample in order to finally decide the matter.
Albert had refused any test, which led to this month's order at the appeals court in Brussels.
The ex-king will now report to an appointed forensic expert who, on the basis of a saliva sample, will carry out a comparative analysis with the DNA of Boel and her mother.
After a request made by Boel, the court said the result of the DNA test would remain secret until the end of the legal proceedings, which could last a year.
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