The rapsheet targets one of Google's most sensitive businesses, as smartphones become by far the dominant player over PCs and laptops.
"Android hasn't hurt competition, it's expanded it," said Kent Walker, senior vice president and general counsel of Google, in a blog.
Google was responding to a long list of charges involving Android that Margrethe Vestager, the EU's outspoken competition commissioner, filed in April.
They include the claim that the firm used practices such as making manufacturers pre-install its market-leading search engine as well as its Chrome browser as the default in their phones.
Google's response comes a week after the company rejected separate EU charges over online shopping and its advertising services in a series of rulings against US companies that has raised hackles across the Atlantic.
The Android charges are seen as especially sensitive for one of Google's most strategic businesses that could alter a global smartphone sector which has taken over traditional PC's as the biggest segment in the world of computing.
The case only pertains to Android-run phones, with the European Commission not considering Apple's iPhone as a factor in the case.
"No manufacturer is obliged to preload any Google apps on an Android phone," Google insisted.
Google is also accused of restricting manufacturers from installing rival versions or modifications of Android, an open source software operating system, on their phones.
The commission, through a spokesman, confirmed the receipt of Google's official response.
"As is standard practice, we will carefully consider Google's response before taking any decision on how to proceed and cannot at this stage prejudge the final outcome of the investigation," it said.
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