The takeover of the al-Qardabiya air base occurred when a militia dispatched to battle the Islamic militants, who already control of much of Sirte, withdrew.
According to Mohammed al-Shamia, spokesman for the so-called 166 Battalion militia, his force retreated after calls for reinforcements from Tripoli went unheeded.
Nearly four years after the ouster of longtime dictator Moammar Gadhafi, Libya is consumed by chaos.
The 166 Battalion militia answers to the umbrella group of Islamist-linked militias that control the capital, Tripoli.
The Tripoli-based factions and militias have so far been hesitant to get into armed confrontation with the Islamic State affiliate which has issued several statements describing them as "infidels" and urging their fighters to defect.
A statement posted by the Islamic State affiliate said the militant group seized the air base along with Gadhafi-era mega-project known as the Great Man Made River.
They have attacked oil fields while trying to expand their territory to the east of Sirte but were repelled by local fighters.
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