During a spacewalk set to begin at 1215 GMT, NASA astronauts Tom Marshburn and Chris Cassidy will try to inspect and possibly repair the ammonia leak that affected the US segment of the orbiting laboratory on Thursday.
Officials said the six-hour spacewalk will set a precedent because it will be conducted on such short notice.
"Good Morning, Earth! A complex & vital day on Space Station, as Tom and Chris suit up and go outside to help fix the ammonia leak," ISS commander Chris Hadfield, who will oversee the spacewalk, tweeted from the space station.
In another message several hours later he said Marshburn and Cassidy were "in the final stages of readying the suits and airlock for their spacewalk."
NASA has stressed that the lives of a multinational crew were not in danger but both Russian and US space experts called the leak "serious."
While in open space, Cassidy will wear a spacesuit with red stripes, while Marshburn's suit will have no stripes, NASA said. Both men have worked together during spacewalks before.
Hadfield will oversee the suit-up of the flight engineers and their duties outside.
On the ground NASA astronaut Terry Virts and European Space Agency astronaut Samantha Cristoforetti conducted a simulated spacewalk in a large pool of water known as the Neutral Buoyancy Laboratory to help refine techniques, the US space agency said.
Norm Knight, NASA chief flight director, said earlier the spacewalk would be "precedent-setting" at the space station because it had to be performed on short notice, adding that it was "probably one of the fastest ones" that the US space agency had had to assemble.
"With such a short time between the detection of the leak and the execution of the contingency EVA, crew timelines were a concern," said space news website Spaceflight101.
NASA said the leak of ammonia, which is used to cool the station's power system, was coming from the same general area as in a previous episode in November last year.
A meteorite or a piece of orbital debris is suspected to have hit the cooling radiator and caused the problem, which International Space Station program manager Michael Suffredini described as an "annoyance because of all the work we have to do to work around the problem.
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