The Southeast Asian countries flanking the Mekong river, which snakes down from the Tibetan plateau to the South China Sea, are among the most biodiverse in the world.
Each year scientists announce scores of new species discovered in the region, which includes Thailand, Myanmar, Cambodia, Laos and Vietnam.
But there are fears that many more species could die out before they are found in the region whose jungle and river ecosystems are increasingly threatened by roads, dams and a thriving illegal wildlife trade.
They include 11 amphibians, two fish, 11 reptiles, 88 plants and three mammals.
"While the global trends are worrisome, and the threats against species and their habitats here in the Greater Mekong are massive, these new species discoveries give us enormous hope," said WWF's Lee Poston.
"But we have to do more to protect their habitat and prevent them from entering the illegal wildlife trade," he added.
The new finds include a bat with a horseshoe-shaped face and a snail-eating turtle discovered by a scientists in a local market in northeastern Thailand.
Although the reptile was first discovered in 2003, it has taken years to confirm its status as a separate subspecies.
Coal mining and pet trade poachers have gravely endangered the lizard, whose numbers are estimated to be fewer than 200, scientists said.
Two new mole species were also found in Vietnam, with researchers noting that their underground dwellings have helped protect them.
Over the past 20 years more than 2,500 new species -- amounting to around two per week -- have been discovered in the Greater Mekong, WWF said.
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