Glazer gained a global profile through his ownership of United, one of the biggest and best supported football clubs in the world.
But he was deeply unpopular with hardcore United fans after loading the club with hundreds of millions of dollars in debt following a 2005 buyout.
His death was announced on the website of the Tampa Bay Buccaneers American football team, one of his other sports franchises.
"The Tampa Bay Buccaneers are saddened to announce the passing of Owner/President Malcolm Glazer earlier this morning at the age of 85," the statement said.
Under Glazer's stewardship of the Buccaneers, the NFL team enjoyed its first Super Bowl success in 2002.
Shares of Manchester United slipped sharply after Glazer's death was announced on the New York Stock Exchange late Wednesday.
Glazer's death is not expected to have any bearing on the ownership of the club, with his family retaining a 90 percent holding in United.
His sons Joel and Avram Glazer continue to serve as co-chairmen of the club. Another son, Bryan, remains on the United board.
Glazer's 2005 takeover of United in a leveraged $1.2 billion buyout triggered widespread protests amongst the club's fans.
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