Willard Wigan, whose works are so small they can fit inside the eye of a needle, has dubbed the creation To See or Not to See.
Smaller than a full stop in a newspaper and only viewable with a microscope, the piece took four weeks to complete.
It is on display at the Light House Media Centre in Wolverhampton, ahead of the anniversary falling on April 23.
Wigan, who was born in Birmingham, said the work "took every ounce of my skill and took a lot out of me".
"The most difficult aspect was getting his proportions right, but I'm really pleased with the result," Wigan was quoted as saying by the BBC.
The micro-Shakespeare has been made from synthetic materials, painted using a floating fibre plucked from the air as a brush.
It is surrounded by an 18-carat Elizabethan-style frame with the words "To see or not to see" underneath.
There will be 30 microscopic pieces on show including "Noddy Holder in a Needle".
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