According to a statement, investigators questioned Netanyahu yesterday for five hours at his Jerusalem residence as part of a corruption probe, the second such interrogation in four days.
The questioning dealt with another affair as well, police said in a statement, adding that a second suspect had been interrogated in the past days.
Netanyahu's office declined to comment.
Netanyahu is suspected of receiving gifts from business people, according to Attorney General Avichai Mandelblit, who is overseeing the investigation. He has provided few other details.
His first questioning on Monday lasted some three hours.
The probe has shaken the country's political scene and raised questions over whether Netanyahu, 67 and in his fourth term as prime minister, would eventually be forced to resign.
US billionaire and World Jewish Congress president Ronald Lauder has been among those questioned in the probe over gifts he allegedly gave Netanyahu and alleged spending on trips for him, reports have said.
Lauder, whose family founded the Estee Lauder cosmetics giant, has long been seen as an ally of Netanyahu.
Netanyahu's office said he had received $40,000 in contributions from Mimran in 2001, when he was not in office, as part of a fund for public activities, including appearances abroad to promote Israel.
Netanyahu has denied any wrongdoing, saying repeatedly that "there will be nothing because there is nothing".
The inquiry has led to fierce debate in Israeli politics, with Netanyahu's allies accusing opposition politicians and some in the media of unfairly pressuring the attorney general.
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