The latest outbreak was detected in three barns containing 43,000 chickens on a farm at Ter Aar, just east of The Hague, the Dutch food and safety watchdog NVWA said.
The outbreak was of the H5 strain, but "it is not clear whether it was of the highly pathogenic variety or not," added the Dutch economic affairs ministry in a letter sent to parliament this afternoon.
The powerful Dutch poultry industry has now been paralysed for a second time this week with a nationwide ban on the transport of all poultry and products since 2.00 pm (1300 GMT) today.
The ban will last up to 72 hours.
Authorities have also thrown a 10 kilometre (6.2 mile) cordon around the farm, with four other farms also being tested for avian influenza.
The chickens are being destroyed and the farm disinfected, the NVWA added.
Dutch officials initially on Sunday banned the transport of poultry around the Netherlands after the discovery of a highly infectuous strain of bird flu following outbreaks of similar strains on the virus in Britain and Germany.
Officials have identified the flu as being the H5N8 strain, previously detected only in Asia.
Some strains of avian influenza are fatal for chickens, and pose a health threat to humans, who can fall sick after handling infected poultry.
But Dutch authorities have said human infection can only occur following "intense and direct contact" with infected birds.
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