From Charleston to suburban Mount Pleasant, they formed a line across the Cooper River to forge what organisers called a Bridge to Peace Unity Chain nearly 4 kilometers long.
"It's not black lives that matter anymore. All lives matter," said Black Lives Matter leader Jay Johnson to loud cheers from a mainly white crowd before the event kicked off.
"We are united as the human race," he said, stripping off his thick dark Black Lives Matter sweatshirt in the early evening heat and humidity.
"This incredible turnout says it all," said organizer Dorsey Fairbairn at the event, where a bagpiper played "Amazing Grace," a chaplain read a prayer, and vehicles crossing the span honked their horns in solidarity.
Once up on the Arthur Ravenel Bridge and holding hands, participants observed nine minutes of silence -- one for each of the victims of Wednesday's bloodbath at Emanuel African Methodist Episcopal Church.
The span, named for a South Carolina politician who once described the NAACP civil rights group as mentally retarded, is the third-longest cable-stayed bridge in the Western Hemisphere.
A 21-year-old white male from South Carolina's interior, Dylann Roof, is charged with nine counts of murder in connection with the Emanuel church shooting, which he reportedly hoped would ignite racial conflict.
Friends and families, both black and white, clapped hands and sang songs along bridge, took souvenir selfies and wrote condolence messages in chalk on the pavement.
They waved at a flotilla of small boats in the river below, hugged each other, exchanged high fives and lifted American flags into the breeze.
The huge turnout contrasted with a protest at a park near the Emanuel church, billed as "a final burial of white supremacy," that attracted only about 40 people.
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