The bus was taking a group of workers from a nearby factory in Nakhon Pathom province west of Bangkok to the southern island of Koh Samui for a holiday trip, according to local police officer Thanachot Shinwongsa.
"Three have been reported dead, all Thais. The number of injuries is not yet confirmed," he told AFP.
"The scene was cleared and the train is back in service."
Video footage of the accident in Nakhon Chaisri district showed the bus slowly crossing the railway when a train ploughed into it at full speed, whipping the vehicle around.
Other cars were seen driving over the tracks alongside the bus just seconds before the collision.
Thailand has the world's second most dangerous roads in terms of per capita deaths.
The World Health Organization estimates about 24,000 people die each year in road traffic accidents.
In January three people were killed and scores injured when a truck crashed into the side of a passenger train several hours south of Bangkok.
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