Andrew Aldridge from auction house Henry Aldridge and Son said the scan proved the instrument was real "beyond reasonable doubt".
Radiographers at BMI Ridgeway Hospital in Wiltshire took a 3D image of the violin to examine it from the inside, BBC News reported.
Wallace Hartley, who came from Colne in Lancashire, and his orchestra, famously played on the violin as the ship sank in the North Atlantic on its maiden voyage from Southampton in 1912.
"The fine detail of the scan meant the auctioneers could examine the construction, interior and the glue holding the instrument together. The scan also assisted in validating the instrument's authenticity," Little said.
In 2006, Henry Aldridge and Son - specialists in Titanic artefacts - was approached by the violin's owner who wanted to sell it, but it needed authenticating.
Over the last seven years, various experts have tried to authenticate the violin, including newspaper archivists, jewellery experts and the forensic science service who found the bodywork still had deposits of salt water.
"The violin was in a heavy duty leather Gladstone type bag, so the violin would have come into contact with water, but it would have been protected by the leather," said Aldridge.
"The instrument is also held together with animal glue, which melts when it is hot, not when it is cold.
"The silver fish plate on the violin along with some other items that was with it points to it being authentic - or an extremely elaborate hoax - so we needed to carry out thorough research and commission the correct experts.
Hartley's body was recovered from the water about 10 days after the ship sank, but the violin was not listed among the inventory of items found with him.
It is claimed the violin survived in a leather case, strapped to Hartley's body, which floated upright in his cork and linen lifejacket.
A diary entry by his fiancee, Maria Robinson, said it was saved from the water in 1912 and returned to her. Following Robinson's death in 1939, the violin was given to her local Salvation Army citadel and was later passed onto the current owner's mother in the early 1940s.
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