"The case of Mr Gross is obviously very important for the government," US delegation head Edward Alex Lee said, stressing that the issue has been and "will be a key point in all our interactions with the Cuban government."
"Our desire is that Mr Gross be freed. We think that Mr Gross should be with his family" in the United States, added Lee.
The acting deputy assistant secretary of state was speaking at a press conference after migration talks with Cuban officials.
The migration talks between the two countries -- which have not had full diplomatic ties since 1961 -- took place in a "frank" and "friendly atmosphere," the two sides said, although there was no indication the strained relations would improve.
This round of talks, the second since the two countries resumed contacts on migratory issues last year, seeks to achieve a "safe, legal and orderly migration between Cuba and the United States," according to US officials.
The Gross case has been the main obstacle Obama has faced in trying to improve relations with the Americas' only communist nation.
Lee said Obama and Secretary of State John Kerry have insisted the United States "wants a new relationship with Cuba" but first Havana must take steps to improve its human rights record.
Every year, thousands of Cubans jeopardize their lives in an attempt to arrive in the US via rudimentary boats and rafts.
Cuba has long opposed the policy, saying it encourages illegal migration.
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