The 18-carat Patek Philippe timepiece is estimated to bring up to USD 150,000 at Christie's on December 17. The stopwatch was made in 1948 and purchased for Dr Kemp Clark by his mother in Switzerland a year later for USD 750.
Clark was one of several attending physicians at Parkland Memorial Hospital in Dallas on November 22, 1963, when the president entered the emergency room. He pronounced Kennedy dead at 1 PM by referring to the watch, according to his family, and signed the death certificate.
Friday marks the 50th anniversary of Kennedy's assassination.
The Reference 1463 Patek was the Geneva-based company's first water-resistant chronograph wristwatch. It is being sold by Clark's direct descendants and comes with its original red clamshell box, sales receipt and Patek Philippe certificate. The back is engraved with the doctor's name.
A portion of the proceeds from the auction will be donated to the Red Cross.
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