A bench of Justice R.K. Agrawal and Justice Abhay Manohar Sapre dismissed the plea by Barun Thakur - father of seven-year-old Pradhuman Thakur, who was murdered on September 8 inside the Gurugram school.
"The petition is dismissed," said Justice Agrawal pronouncing the order.
Ryan group of school's Founder-Chairman Augustine F. Pinto, his wife and Managing Director Grace Pinto and their son and CEO Ryan Pinto were granted anticipatory bail by the Punjab and Haryana High Court on November 21.
Pradhuman Thakur, a Class 2 student was found dead in the washroom with his throat slit minutes after his father had dropped him at the school.
The High Court while granting the anticipatory bail to the Pintos had asked them to join the investigation in the case.
The court had barred them from leaving the country without its permission.
Initially the Haryana Police was investigating the case and had arrested bus conductor Ashok as the main accused in the murder of Pradhuman.
However, later the case was transferred to the Central Bureau of Investigation (CBI), which arrested a Class 11 student of the same school as the prime accused.