"At least 19 Al-Nusra Front (jihadist) fighters and 10 Kurds have been killed since the day before yesterday in clashes in the oil region of Hassakeh," the NGO said.
Yesterday, the group said Syrian Kurdish fighters had pushed members of al-Nusra and the al-Qaeda-affiliated Islamic State of Iraq and the Levant out of the town of Ras al-Ain and its nearby border crossing with Turkey.
The clashes between Kurdish fighters and jihadists erupted after Al-Nusra Front militants attacked a convoy of Kurdish women fighters, Observatory director Rami Abdel Rahman said.
In the early days of the Syria conflict, when the opposition was desperate for help from any quarter, jihadist fighters were welcomed but a spate of abuses has fuelled a major backlash.
The Observatory said that jihadist fighters began firing rockets at Ras al-Ain, in western Hassakeh, after their expulsion.
They also attacked several roadblocks manned by Kurdish fighters and clashes were ongoing in the village of Tall Alu and Karhok in eastern Hassakeh, the group added.
Syria's Kurdish minority have walked a sometimes ambiguous line in the country's conflict, which is now in its third year.
Despite occasionally cooperating with rebel fighters, the country's Kurds have largely chosen to remain outside the conflict, and have sought to keep both regime troops and rebels out of their areas.
Their position has earned them the ire of some rebels, who fault them for failing to back the uprising.
And the community's more liberal interpretation of Islam has also made it a target for some extremist rebel groups, including Al-Nusra and the Islamic State of Iraq and the Levant.
The group said at least four missiles fired by regime war planes hit residential buildings, killing five civilians and injuring dozens more.
At least 120 people were killed throughout Syria on Wednesday, according to the group, including 42 civilians, 61 rebels and 17 soldiers.
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