The workers from Bangladesh, India, Pakistan and Nepal have been left without accommodation and money, said the head of Qatar's National Human Rights Committee.
"There are a lot of migrant workers affected by this decision," said committee chairman Ali bin Smaikh Al-Marri.
Many of those affected were farmers who drive livestock between the two countries, said Marri.
"Usually the workers travel with Qataris -- many Qataris employ farmers and travel with their domestic workers and drivers," he told a news conference.
"They have no shelter and cannot access money."
On June 5, Saudi Arabia, the United Arab Emirates, Egypt and Bahrain announced they were suspending all ties with Qatar over accusations the emirate supports extremist groups -- a claim Doha denies.
The four states closed their airspace to Qatari carriers and sealed off the emirate's only land border with Saudi Arabia, a vital route for its food imports.
They also ordered all Qataris to leave and their own nationals to return home.
Some 12,000 camels and sheep are reportedly being forced to trek back to Qatar from Saudi Arabia because of the crisis.
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