Erdogan had sought a court order to stop the Axel Springer media group's chief Mathias Doepfner from repeating support for a TV satirist who crudely insulted the Turkish leader.
After failing to get an injunction from a lower court last month, Erdogan also lost an appeal before the higher regional court in the western German city of Cologne.
The judges said they considered Doepfner's letter of support "a permissible expression of opinion as protected under Article 5" of Germany's constitution, the court said in a statement.
The legal action came after Doepfner published in April an open letter in one of the Springer group's newspapers, in which he backed Jan Boehmermann - the satirist who in a poem accused Erdogan of bestiality and watching child pornography.
Boehmermann's recital of his so-called "Defamatory Poem" on national television in late March sparked a diplomatic firestorm and a row over freedom of expression.
During the broadcast Boehmermann gleefully admitted his poem flouted Germany's legal limits to free speech and was intended as a provocation.
In his letter, Doepfner took the comedian's side, declaring: "For me, your poem worked. I laughed out loud."
The higher regional court stressed today that its ruling in favour of Doepfner had no bearing on the other case still pending.
Erdogan has come in for fierce Western criticism of late over his increasingly authoritarian rule, just as the European Union has turned to Turkey to help stem the influx of asylum seekers from Middle East war zones.
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