The report by the newsweekly Der Spiegel comes after an investigation into alleged US snooping on Chancellor Angela Merkel's mobile phone was dropped in June due to lack of proof.
German-US relations were badly strained after fugitive US intelligence contractor Edward Snowden revealed mass US surveillance around the world in 2013.
The latest probe by Germany's federal prosecutors targets persons unknown for "espionage activities", Der Spiegel said.
It centres on the personal laptop of a department chief in the chancellery onto which a spying virus known as "Regin" was allegedly installed, the magazine said.
"We can confirm that there is an inquiry" relating to "malicious software" called Regin, a spokeswoman for the federal prosecution service told AFP, declining to confirm other details from the Spiegel report.
The magazine, citing specialists, said there "is no doubt" that Regin can be linked to the NSA or Britain's GCHQ eavesdropping agency, which was revealed in Snowden's documents to have worked closely with its US equivalent, including in the interception of German phone calls and emails.
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