The new fund, which received financing from existing investors as well as new ones, is ten times larger than its first fund raisedin 2012, it said in a statement.
The company got Ratan Tata on board as a special advisor last year.
"It will largely target Series A and select Series B investments primarily in Southeast Asia, as well as, on a more opportunistic basis, businesses in India and Australia particularly where such businesses may benefit from expansion into Southeast Asia," it added.
"This is a significant milestone for Southeast Asia's fast growing startup ecosystem... With an exceptional team of 15 investing and operational professionals we intend to continue to invest and help build the best technology companies from this region," Jungle Ventures Founding Partner Amit Anand said.
Since inception in 2012, Jungle is amongst the few VCs that have successfully exited companies via M&As to global brands like Twitter, HomeAway, Rakuten and Shopclues.
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