The pilots' union Vereinigung Cockpit has called for a six-hour walk-out between 5:00 pm and 11:00 pm (1500-2100 GMT) over plans to change the airline's early retirement policy.
The industrial action is focussing on the country's biggest airport in Frankfurt and will ground most domestic and regional flights.
Lufthansa said individual flights would already be cancelled as early as 1:30 pm (1130 GMT) and most flights from 4:00 pm (1400 GMT).
The airline promised to try and limit the impact of the strike for customers.
Last Friday more than 100 flights were cancelled at several of Germany's main airports as pilots of Lufthansa's low-cost airline Germanwings also walked out.
And on Monday, a union for German train drivers called for a three-hour walkout to turn up the pressure on management of the national rail operator Deutsche Bahn in gridlocked wage talks.
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