Francis also pleaded for divine aid to rescue child soldiers "robbed of their childhood" and for peace in the conflict-torn Central African Republic which he said was "often forgotten and overlooked".
In a wide-ranging address known as the "Urbi et Orbi" (To the City and to the World) blessing that touched on many conflicts, the Argentine pope invited non-believers to join in a "desire" for peace in the world.
"Let us continue to ask the Lord to spare the beloved Syrian people further suffering, and to enable the parties in conflict to put an end to all violence and guarantee access to humanitarian aid," he said.
The conflict in Syria is estimated to have killed more than 126,000 people since it first started out as peaceful anti-regime protests in 2011 and the violence there has unsettled the Middle East as a whole.
"Heal the wounds of the beloved country of Iraq," the pope said in his prayer.
In his weekly address, US President Barack Obama stressed this year marks the first time in years that many US troops and recent veterans have spent Christmas at home with their families.
"For many of our troops and newest veterans, this might be the first time in years that they've been with their families on Christmas," he said. "In fact, with the Iraq war over and the transition in Afghanistan, fewer of our men and women in uniform are deployed in harm's way than at any time in the last decade."
The first Latin American pope asked for "social harmony in South Sudan, where current tensions have already caused numerous victims and are threatening peaceful coexistence in that young state".
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