"The provisional size of the UK and Gibraltar electorate indicates a UK record of 46,499,537," the Electoral Commission said in a statement today.
The previous record was in last year's general election when the number was 46,354,197.
As with British parliamentary elections, Irish citizens living in Britain -- a population estimated at around 500,000 people -- are allowed to vote.
Commonwealth citizens, including nationals of European Union member states Cyprus and Malta, can also vote.
The deadline for registering was June 7 but this was extended by two days after a rush of last-minute sign-ups caused the official registration website to crash.
Around 132,000 of the 525,000 people who did successfully register on June 7 were aged under 25, compared to around 13,000 from the 65 to 74 age group.
Opinion polls have shown a clear generational divide, with younger voters more in favour of staying in the EU while older voters tend to favour a Brexit.
Younger people are also traditionally seen as less likely to vote, meaning that high registration numbers could favour the "Remain" side.
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