A defence official in Washington told AFP that American forces conducted "about 10" strikes in Yemen today.
The attacks hit similar targets as yesterday -- weapons caches, fighters and military equipment, the official said.
The Pentagon said yesterday it had carried out more than 20 strikes against Al-Qaeda in the southern provinces of Shabwa and Abyan and the central province of Baida.
Yemeni officials said at least 12 suspected militants were killed in those strikes, which came barely a month after a botched American raid against the group left multiple civilians and a US Navy SEAL dead.
Security officials in the area said eight jihadists were killed, and tribal sources said that women and children were wounded.
The valley is a jihadist stronghold and was also one of the targets of the previous day's strikes.
Jihadists retaliated with anti-aircraft fire, security officials and tribal sources said, adding that US helicopters took part in the operation.
One resident said it had been a "terrifying night".
US President Donald Trump faced broad criticism at home after he authorised the January 29 commando raid during which Navy SEAL Ryan Owens and many civilians were killed.
The US official said today the latest targets had been acquired long before the January 29 raid, but that some intelligence gathered then corroborated what was already known about the new targets.
"What we already knew about these sites was reconfirmed," the official said.
Al-Qaeda has exploited a power vacuum created by two years of war between Yemen's government and Shiite rebels who control the capital to consolidate its presence, particularly in the south and east.
He said yesterday's strikes targeted Al-Qaeda militants, equipment and infrastructure. Another US official said they had involved both fighter jets and drones.
Successive American administrations have kept up a drone war against Al-Qaeda in Yemen since soon after the September 11, 2001 attacks.
Washington regards the Yemen branch to be the jihadists' most dangerous, and holds it responsible for several plots to carry out attacks in the West.
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