In the biggest backing yet for the "Remain" camp, 1,280 business leaders, which included representatives of 51 FTSE 100 companies, signed a letter warning that Brexit - or Britain's exit from the EU - would mean "economic uncertainty and put jobs at risk".
Their warning came on the last official day of campaigning before polling booths open at 7 am local time tomorrow with the final result expected early on Friday.
But the 'Britain Stronger in Europe' (Remain) camp described today's letter as "unprecedented" as it included around 900 small businesses, ranging from a salt maker in Anglesey, dairy farmers in Devon, printers in Antrim and whisky distilleries in the Scottish Highlands.
Their letter reads, "Britain leaving the EU would mean uncertainty for our firms, less trade with Europe and fewer jobs. Britain remaining in the EU would mean the opposite - more certainty, more trade and more jobs. EU membership is good for business and good for British jobs. That's why, on 23 June, we back Britain remaining in the EU".
Speaking to the BBC, he said "We are not shackled to a corpse. You can see the European economy's recovery. It's the largest single market in the world.
"The idea you have to choose between being a success in the European single market of 500 million and campaigning to have jobs and wealth created by trading with other economies - you don't have to choose, you should do both.
Making a personal plea to those who fear greater European control, he described himself as a "deeply patriotic person".
"We're out of the euro, out of ever-closer union, we're
proud Brits and really proud of what our country can achieve, but, frankly, we achieve more if we're in these organisations fighting for British interests and British values rather than standing outside for them," Cameron said.
It saw Vote Leave's star campaigner - former London mayor Boris Johnson - clash with his successor Sadiq Khan and Scottish Conservative colleague Ruth Davidson.
Johnson declared in his closing statement that Thursday could become "our country's Independence Day", adding, "The ideal position for us is to take back control tomorrow - of huge amounts of money, so we can spend it on our priorities. Take back control of our immigration system, take back control - fundamentally - of our democracy.
"The problem is this, Boris. You might start off by saying how wonderful immigration is. But your campaign hasn't been Project Fear, it's been Project Hate as far as immigration is concerned," he told Johnson.
Meanwhile, polling booths up and down the UK are preparing for the big day.
Though voting willclose at 10 pm UK time tomorrow, the future of Britain's relationship with the EU willnot be known until at least nine or 10 hours later, at around 7 am local time on Friday.
In a departure from the norm, no major broadcasters have commissioned any exit polls over concerns about accuracy following the fiasco of the 2015 General Election which had wrongly predicted a hung Parliament.
The resultwillbe declared by Jenny Watson, the chair of the UK's Electoral Commission and thereferendum's chief counting officer at Manchester Town Hall on Friday morning.
Thunderstorms and flooding is forecast in many parts of the UK, which could affect turnout.
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