Delhi Auto Rickshaw Sangh and Delhi Pradesh Taxi Union said that the new scheme will "snatch" the livelihood of the drivers of auto rickshaws and taxis.
On September 2, several auto and taxi unions had also gone on a day-long strike against this scheme.
"We will not sit silent till our demand is met. We have decided to take out protest march with black flags against the AAP government for introducing 'City Taxi' scheme.
Soni, who is also general secretary of Delhi Pradesh Taxi Union, said that they cannot compromise with the livelihood of drivers and if there is any need in future, they will launch a series of protests.
According to the 'City Taxi' scheme, vehicles having capacity of 600 cc onwards can register with the government. Until now, only those vehicles which had capacity of 750 cc or more were issued permits.
