Chanting "Death to the enemies of Afghanistan!" and "Death to Daesh!", the Arabic acronym for IS, the protesters in the city of Herat marched on the governor's office, carrying pictures of Shiites killed in recent attacks.
"Daesh attacks on our mosques are increasing everyday. They want to create a rift between Shiites and Sunnis," Qurban Ali, a 40-year-old demonstrator, told AFP.
"This is a dangerous trend and we want the government to protect us."
Until a few months ago IS was largely confined to the eastern province of Nangarhar bordering Pakistan, where it is notorious for brutality including beheadings despite a US-backed offensive against the group.
But the government this week said the group was steadily expanding into other provinces.
"Our initial information shows Daesh is behind the recent attacks in Herat. They are expanding and are always looking for new geographical areas," Najeebullah Mani, head of counter-terrorism at the interior ministry, told reporters in Herat.
Herat, which borders Shiite-dominated Iran, has recently witnessed a surge in attacks on the minority group's mosques.
A prayer leader was killed and five others wounded in one such attack on Sunday. The group did not officially claim responsibility for the assault.
"We will not allow Daesh to make Afghanistan another Syria," said 30-year-old Jawad, another protester. "The government must come up with a plan to protect Shiites."
Last year Afghanistan witnessed a wave of attacks on Shiites claimed by IS, which considers Shiite Muslims apostates.
In July the IS militants targeted members of Kabul's Shiite community in a suicide bombing that killed more than 80 people and wounded 130.
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