WADA's foundation board unanimously passed a recommendation from a committee on Wednesday which found RUSADA non-compliant with the global body's charter.
The decision had been anticipated following a damning report by a WADA independent panel which uncovered a state-sponsored doping program in Russia.
RUSADA had been heavily implicated in the scandal and was accused of destroying samples requested for analysis by WADA.
The International Association of Athletics Federations (IAAF) had already provisionally suspended Russia from international competition last week but left the door open to a return in time for the Olympics.
Under rules agreed by WADA and the International Olympic Committee (IOC) only countries in full compliance with the anti-doping agency's code can participate in the Games.
Russia has vowed to follow a roadmap laid down to overhaul its drug-testing program in order to avert the prospect of an Olympic ban -- a nuclear option that neither the IAAF nor the IOC wants to see.
WADA President Craig Reedie insisted however that the agency would move to ensure a "watertight" anti-doping regime in Russia.
"Make no mistake, we will not rush this process of compliance, we will do it right -- the integrity of sport is under threat," Reedie said in a statement.
=======================
However the prospect of Russia being offered a straightforward route back into the international fold has met with opposition in some quarters.
On Wednesday, Olympic legend Ed Moses added his voice to calls for Russian track and field athletes to be barred from next year's Olympics over the scandal.
"The only sanction is that enough is enough. It is to state loudly and clearly that the Russian athletics team cannot go to Rio," Moses told the WADA meeting in Colorado Springs.
Moses said he wanted to see an investigation into all
sports in Russia before they could be deemed compliant.
Moses calling for a ban echoed the position taken by leading anti-doping bodies in a statement on Monday, who said Russian track and field stars must be banned from Rio.
"The corruption in Russian Athletics deserves no less," the Institute of National Anti-Doping Organisations said. "A strong deterrent message must be sent."
- 'Pivotal moment' -
====================
Beckie Scott, the former Canadian cross-country skier who chairs WADA's athlete committee, backed Moses call for a wider investigation into doping in Russian sport.
"It's a pivotal moment for WADA. There are a lot of athletes watching and waiting who are counting on the forces of anti-doping to bring their full strength and resolve to this fight."
WADA member Dick Pound, who chaired the investigation into the scandal, expressed sympathy with athletes seeking a ban, but stressed Russia needed to be given an opportunity to change its ways.
"The objective is to change behavior, not to go out and do an eye-for-an-eye, and do a punitive thing," Pound told reporters.
"I understand why they feel like that but it might not be the right solution. We've got to see how Russia responds to this.
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