The charred piece of parchment from the sixth century AD was found in the ashes of an ancient synagogue at Ein Gedi, on the shores of the Dead Sea, in 1970 but until now has been impossible to read.
"The most advanced technologies allowed us to virtually unroll a scroll, part of a bible, from about 1,500 years ago," said Pnina Shor of the Israel Antiquities Authority (IAA).
"After the Dead Sea Scrolls, this is the most significant find of a written bible," she told journalists.
"We tried to read it right after, but with no success," he said.
"We didn't know what was hidden there with the technology of the time."
The fragment measuring seven centimetres (two and three-quarter inches) in length resembles a peace of charcoal.
It has now been found to contain the first eight verses of the Old Testament book of Leviticus, which lay down the rules for ritual sacrifice, said Shor, head of the IAA's Dead Sea Scrolls projects.
Kept in the IAA's dark, climate-controlled vaults along with the Dead Sea Scrolls themselves, the parchment remained a closed book until last year when Israeli company Merkel Technologies volunteered the use of its micro-CT scanner.
Shor said she believed the complete scroll would have contained the whole of the Torah, the Jewish bible, but Kentucky University professor Brent Seales said it was too early to know if any more information could be retrieved from the Ein Gedi parchment.
"We're really not sure. It's such a technical challenge," he told the news conference through a video link. "We know there is more though."
Shor said the discovery filled an important gap in between the Dead Sea Scrolls, written more than 2,000 years ago, and the 10th century Aleppo Codex.
The oldest of the documents dates to the third century BC and the most recent to about 70 AD, when Roman troops destroyed the Second Jewish Temple in Jerusalem.
The Aleppo Codex, was written in the Galilee in the 10th century AD and named for the Syrian city to which it resided from the late 14th century until it was smuggled to Israel in the 1950s.
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