Apparently not.
In an unusual trial, close to 100 per cent of people described her expression as unequivocally "happy", researchers revealed today.
"We really were astonished," neuroscientist Juergen Kornmeier of the University of Freiburg in Germany, who co-authored the study, told AFP.
Kornmeier and a team used what is arguably the most famous artwork in the world in a study of factors that influence how humans judge visual cues such as facial expressions.
Known as La Gioconda in Italian, the Mona Lisa is often held up as a symbol of emotional enigma.
Using a black and white copy of the early 16th century masterpiece by Leonardo da Vinci, a team manipulated the model's mouth corners slightly up and down to create eight altered images -- four marginally but progressively "happier", and four "sadder" Mona Lisas.
A block of nine images were shown to 12 trial participants 30 times.
In every showing, for which the pictures were randomly reshuffled, participants had to describe each of the nine images as happy or sad.
Instead, "to our great astonishment, we found that Da Vinci's original was... Perceived as happy" in 97 per cent of cases.
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