Ditko, who co-created Spider-Man and Doctor Strange with comic book legend Stan Lee, was found dead in his apartment on June 29. He was 90.
Holland, 22, took to Twitter to honour the comic book artiste, saying Ditko made "many people happy and changed lives".
"We all want to leave our mark on the world - this guy crushed it. He made so many people so happy and changed lives - most of all, mine! Thank you Steve - your life lives on man, thank you," he wrote.
His latest turn as Spider-Man came in the blockbuster "Avengers: Infinity War", which released this April.
Marvel Entertainment also posted a tribute to Ditko.
The company shared a picture of Spider-Man and wrote, "Thank you, Steve. Rest in Peace."
Marvel Entertainment's president, Dan Buckley, condoled Ditko's death in a statement released on the company's website.
"Today, the Marvel family mourns the loss of Steve Ditko. Steve transformed the industry and the Marvel Universe, and his legacy will never be forgotten. Our thoughts are with his family, loved ones, and fans during this sad time," Buckley said.
(This story has not been edited by Business Standard staff and is auto-generated from a syndicated feed.)