"We have already said that we are absolutely not familiar with this whole story," said Kremlin spokesman Dmitry Peskov.
"We never had any contact with this lawyer, that is why we are not in the loop and have nothing to say. She doesn't have even the slightest relation to us."
The Kremlin had on Monday denied links to the lawyer.
The June 2016 meeting with lawyer Natalia Veselnitskaya has thrust Donald Trump Jr into the centre of a growing scandal over whether Trump's associates colluded with Moscow to tilt the 2016 election in the Republican's favour.
A string of emails Trump Jr released yesterday showed he agreed to the meeting after claims from a British music promoter that a senior official in Moscow had offered to pass on dirt on Hillary Clinton and Veselnitskaya was a "Russian government attorney".
Peskov ridiculed claims that Veselnitskaya was working for the Russian government as "inappropriate and absurd".
Veselnitskaya herself has also denied she had any damaging information on Hillary Clinton or that she is tied to the Russian authorities.
The Kremlin has repeatedly denounced accusations it meddled in the US election to help get Donald Trump to the White House and Peskov said the allegations resembled a "drawn-out TV series."
US intelligence agencies have accused Russian President Vladimir Putin of approving a hacking and influence campaign to sway the vote.
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