Perry and Musgraves opened the special tribute with a duet of 'Here You Come Again' in matching red jumpsuits. Both the costumes were inspired by Parton's outfits from the 1970s and 1980s.
Halfway through the performance, Parton joined Perry and Musgraves onstage, dressed in a white lace gown to finish the song.
They were then joined by Morris, who sang 'After the Gold Rush'. Parton later thanked Cyrus and Morris and sent them offstage.
She then welcomed onstage country group 'Little Big Town' to perform 'Red Shoes', which is a new song that Parton co-wrote with Linda Perry for the Netflix rom-com 'Dumplin' and the whole group came together at the end to sing Parton's biggest hit '9 to 5'.
The star-studded crowd was seen enjoying and cheering during the performance.
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