Rane told reporters that he had submitted his resignation to the Speaker's office and it had been accepted.
"I have been told by my party workers and constituents to resign from the Congress, which meant I had to resign as MLA too," Rane said.
Earlier, Rane went missing from the assembly during the vote on the confidence motion moved by Chief Minister Manohar Parrikar in the assembly even as Congress leader Chandrakant Kavlekar claimed he could not be traced.
Over the last few days, Rane had been accusing the Congress leadership, including state president Luizinho Faleiro and Congress General Secretary Digvijaya Singh, of sabotaging the party's attempts to come to power though the Congress emerged as the single largest party in the February 4 polls.
Rane's father, Pratapsingh Rane, is also a Congress legislator and a former Chief Minister.
Responding to Rane's allegations, Singh had claimed that the MLA was in touch with Chief Minister Manohar Parrikar.
(This story has not been edited by Business Standard staff and is auto-generated from a syndicated feed.)