Gandhi arrived at the court in Bhiwandi at 11.10 am amid heavy security cover and was greeted by hordes of people raising slogans in his support.
Inside the court, when Judge A I Sheikh, read out charges against him, Gandhi said, "I am not guilty."
The court had on May 2 asked Gandhi to appear before it today to record his plea in the defamation case filed by RSS worker Rajesh Kunte in 2014.
Kunte had filed the case after watching Rahul Gandhi's speech at an election rally in which he had alleged that the RSS was behind the killing of Mahatma Gandhi.
(This story has not been edited by Business Standard staff and is auto-generated from a syndicated feed.)