The WTO's highest court overturned an earlier judgement from 2018, handing Seoul a final victory in a legal battle that has dragged on for years.
"Even though the ruling did not acknowledge that South Korea's measures comply with the WTO rules, it is extremely regrettable that Japan's argument was not approved," said the foreign ministry in Tokyo.
"There is no change in Japan's position of demanding South Korea lift all the restriction measures, and we will pursue this via talks with South Korea," added the ministry in a statement.
Tokyo first took the row to the WTO in May 2015, requesting consultations -- the first step under the global trade body's settlement system.
But talks broke down, prompting Japan to seek a WTO ruling in August 2015.
The WTO panel ruled in 2018 that South Korea should lift its ban but the so-called appellate court quashed this, the final word on the subject.
But the majority of other countries and regions -- including the European Union -- have lifted bans on imports or allow imports on condition that a certificate of inspection is attached.
(This story has not been edited by Business Standard staff and is auto-generated from a syndicated feed.)