He reached the agency's office at Jamnagar House in central Delhi at about 10:30 AM along with his lawyers, they said.
This is a scheduled appearance of Vadra before the investigating officer (IO) of the case in a criminal complaint related to allegations of money laundering to purchase assets abroad.
He had skipped the summons Tuesday, citing bad health.
Vadra has been questioned by the ED in this case for 23 hours over three days of deposition earlier this month.
He has been asked by a Delhi court to cooperate in the probe being conducted by the central probe agency.
The ED case against Vadra relates to allegations of money laundering in the purchase of a London-based property located at 12, Bryanston Square worth 1.9 million GBP (British pounds), which is allegedly owned by him.
The agency had told the court that it has received information about various new properties in London which belong to Vadra. These include two houses, one worth 5 million GBP and the other valued at 4 million GBP, six other flats and more properties.
Vadra has denied the allegations of possessing illegal foreign assets and termed them a political witch hunt against him. He said he was being "hounded and harassed" to subserve political ends.
Vadra's statement is being recorded under Section 50 (powers of authorities regarding summons, production of documents and to give evidence) of the Prevention of Money Laundering Act (PMLA).
His first appearance before the ED earlier this month acquired political overtones after his wife Priyanka Gandhi, recently appointed Congress general secretary in-charge of eastern Uttar Pradesh, accompanied him to the investigating agency's office on one day while she picked him up after questioning the next time.
Vadra had similarly deposed before the ED twice in Jaipur in another money-laundering case related to an alleged land scam in Bikaner.