The earlier law stipulated that minimum penalty for throwing waste was Rs 50 and the maximum fine was Rs 5,000.
It also came on a day, state Urban Development Municipal Affairs Minister Firhad Hakim was elected the new mayor of Kolkata after the exit of Sovan Chatterjee, on the directions of Chief Minister and TMC chief Mamata Banerjee.
Banerjee had formed a 11-member committee on Wednesday to check spitting and littering in public places.
Hakim said, he will take up a major awareness drive to keep the city clean.
A senior citizen, who did not wish to be named, said "When the city had earlier failed to impose a fine of Rs 50 for littering, I am not sure how effective it will be this time.