Interim coach Lionel Scaloni named the five-time World Player of the Year in his squad for the friendlies against Venezuela and Morocco.
Another long-term absentee, PSG's Angel Di Maria, also makes the squad.
In all, Messi has missed six friendlies since the World Cup in Russia but many believe he will be an essential part of the team that travels to Brazil for the Copa America, which kicks off June 14.
He was on the losing side in major finals three years running from 2014-16, first to Germany in the World Cup in Brazil, then twice to Chile on penalties in the Copa America, including the one-off Centenary edition of the competition.
With 65 goals in 128 appearances, Messi is Argentina's all-time top goalscorer. He's also Barca's top marksman ever with more than twice the number of goals as the next best.
But he's never managed to match his club form when operating on the international stage.
Argentina play Venezuela in Madrid on March 22 and then travel to Tangier four days later to tackle hosts Morocco.