This is Chennaiyins debut AFC Cup campaign following their 2017-18 ISL title success and are placed along with Colombo FC in the South Asian Zone of the competition, a press release said.
Chennaiyin is also the first ISL outfit to participate in the AFC Cup, with this qualifying tie against Colombo FC for a place in the group stage of the 2019 edition.
Chennaiyin concluded it's 2018-19 ISL campaign with a 1-0 defeat to FC Goa last week, with the focus now shifting to their maiden AFC Cup campaign.
Chennaiyin players arrived in Colombo on Monday evening from Goa, having extended their stay there to prepare for the AFC Cup qualifying playoff against the Sri Lankan side.
"The boys are in good spirits, having arrived in Sri Lanka ahead of what is a very big game for us against Colombo FC.
Australian Chris Herd, who joined CFC in the January transfer window, will be the club's Asian player in the qualifier with the Brazilian trio of Mailson Alves, Eli Sabia and Raphael Augusto being the other three foreigners.
Colombo had defeated Bhutans Transport United 9-2 on aggregate in the preliminary round to set up the clash with Chennaiyin.
The second leg of the qualifying playoff will be played in Ahmedabad on March 13.