A molecule, used to enhance the flavour in soy sauce, can help develop compounds that are 70 times more potent than Tenofovir, a first-line HIV drug therapy, scientists say. For human immunodeficiency virus (HIV) patients being treated with anti-AIDS medications, resistance to drug therapy regimens is commonplace.
Often, patients develop resistance to first-line drug therapies, such as Tenofovir, and are forced to adopt more potent medications. Virologists at the University of Missouri are testing the next generation of medications that stop HIV from spreading, and are using a molecule related to flavour enhancers found in soy sauce to develop compounds that are more potent than Tenofovir. "Patients who are treated for HIV infections with Tenofovir, eventually develop resistance to the drugs that prevent an effective or successful defense against the virus," said Stefan Sarafianos, associate professor of molecular microbiology and immunology in the University of Missouri School of Medicine, and a virologist at the Bond Life Sciences Center at MU. "EFdA, the molecule we are studying, is less likely to cause resistance in HIV patients because it is more readily activated and is less quickly broken down by the body as similar existing drugs," Sarafianos said. In 2001, a Japanese soy sauce company inadvertently discovered the EFdA molecule while trying to enhance the flavour of their product. The flavour enhancer is part of the family of compounds called 'nucleoside analogues' which is very similar to existing drugs for the treatment of HIV and other viruses. EFdA samples were sent for further testing, which confirmed EFdA's potential usefulness against HIV and started more than a decade of research. EFdA, along with eight existing HIV drugs, is part of the class of compounds called nucleoside reverse transcriptase inhibitors (NRTIs). NRTIs 'hijack' the HIV replicating process by 'tricking' building blocks inside the virus. Since EFdA appears similar to those building blocks, the virus is misled into using the imposter, which prevents HIV replication and halts the spread of the virus. In their latest study, Sarafianos and his colleagues, including researchers from the University of Pittsburgh and the National Institutes of Health, helped define how EFdA works on a molecular level. Using virology techniques and nuclear magnetic resonance spectroscopy (NMR), they pieced together the exact structure and configuration of the molecule. Compounds developed by Sarafianos and his team currently are being tested for usefulness as potential HIV-halting drugs with pharmaceutical company Merck. "EFdA works extremely well on HIV that is not resistant to anti-AIDS drugs, it also works even better on HIV that's become resistant to Tenofovir," Sarafianos said.