Kane has been out of action since April 9 due to an ankle injury, resulting in a premature end to his Premier League efforts in 2018-19.
"The Three Lions have a number of players still involved with their clubs, with the Emirates FA Cup, Champions League, and Europa League Finals all scheduled to take place over the next few weeks," the Football Association of England said in an official statement.
"And that means players will be reporting to St. George's Park for training at different times before a final squad of 23 will head to Portugal ahead of the semi-final with the Netherlands in Guimaraes on Thursday 6 June and a second game on Sunday, June 9," it added.
Players from Manchester City will be involved in the FA Cup final against Watford on May 18 so that they would join England's training camp in the week commencing from May 27.
Liverpool, Tottenham Hotspur, and Chelsea are all involved in the UEFA finals next month so that players from those clubs are expected to join the group in the week commencing from June 3 before the squad travels to Portugal for the Nations League finals.
England's 27-man squad is as follows:
Defenders: Trent Alexander-Arnold (Liverpool), Danny Rose (Tottenham Hotspur), Kieran Trippier (Tottenham Hotspur), Ben Chilwell (Leicester City), Joe Gomez (Liverpool), Michael Keane (Everton), Harry Maguire (Leicester City), John Stones (Manchester City), Kyle Walker (Manchester City)
Midfielders: Dele Alli (Tottenham Hotspur), Eric Dier (Tottenham Hotspur), Jesse Lingard (Manchester United), Nathan Redmond (Southampton), James Ward-Prowse (Southampton), Ross Barkley (Chelsea), Fabian Delph (Manchester City), Jordan Henderson (Liverpool), Declan Rice (West Ham United), Jadon Sancho (Borussia Dortmund), Harry Winks (Tottenham Hotspur)
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