Officials said Jain arrived at the ED office here this morning for the second round of questioning.
He was first questioned in the case last week.
The PMLA case was registered by the ED in August last year on the basis of a CBI FIR.
The ED probe will focus if the minister and other accused in the case created illegal assets by laundering the alleged tainted funds.
The Central Bureau of Investigation (CBI) complaint had said Jain could not explain the source of funds received by four companies in which he was a shareholder.
It had registered a case against him, his wife and four others on charges of money laundering.
The CBI has also questioned him in the case earlier.
It had said that Rs 4.63 crore was allegedly received through Prayas Info Solutions, Akinchan Developers, Managalyatan Projects and Indo-Metal Impex Pvt Limited in 2015-16.
They had said Jain and his wife were allegedly holders of one-third of the shares in these companies during the period.
The CBI has alleged that Jain had control over these companies either in the form of being one of the directors and by holding one-third of shares of these companies in his name or in the names of his family members or others.
The probe agency had also claimed that these were shell firms used for parking money in the form of investments in equity shares in connivance with Kolkata-based shell companies.
"This apart, before becoming a public servant, he was allegedly involved in laundering Rs 11.78 crore during 2010-12 through these companies as well as other firms also based in New Delhi," the CBI had said.
The Aam Aadmi Party (AAP) had refuted the CBI's allegations and said that reports of Jain's involvement in shell companies and benami land deals were "baseless".
Jain, also the minister for home, PWD, industries and urban development, is an MLA from the Shakurbasti constituency.
(This story has not been edited by Business Standard staff and is auto-generated from a syndicated feed.)