The 26-year-old, who missed the trip to Australia after being suspended from playing for England, appeared at a magistrates' court in the southwestern city of Bristol along with Ryan Ali and Ryan Hale.
It follows the altercation during the early hours of September 25 last year, several hours after England had played a one-day international against the West Indies in the city.
Ali, Stokes and Hale spoke to confirm their names, dates of births, addresses and nationalities. The clerk read out the charges and all three defendants indicated not guilty pleas.
Stokes is accused jointly with Ali and Hale of using or threatening unlawful violence towards another.
The charge states that his "conduct was such as would cause a person of reasonable firmness present at the scene to fear for his personal safety".
The other two defendants face the same charge.
All three defendants elected to be tried by a jury at a crown court.
Without Stokes, England lost the Ashes to Australia 4-0.
(This story has not been edited by Business Standard staff and is auto-generated from a syndicated feed.)